Resistance and co-resistance of metallo-beta-lactamase genes in diarrheal and urinary-tract pathogens
Microorganisms, 2024
Abstract
Carbapenems are the antibiotics of choice for treating multidrug-resistant bacterial infections. Metallo-β-lactamases (MBLs) are carbapenemases capable of hydrolyzing nearly all therapeutically available beta-lactam antibiotics. Consequently, this research assessed the distribution of two MBL genes and three β-lactamases and their associated phenotypic resistance in diarrheal and urinary-tract infections (UTIs) to guide future policies. Samples were collected through a cross-sectional study, and β-lactamase genes were detected via PCR. A total of 228 diarrheal bacteria were isolated from 240 samples. The most predominant pathogens were(32%) andspp. (7%). Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 50.4%, 65.6%, 66.8%, 80.5%, 54.4%, 41.6%, 25.7%, 41.2%, 37.2%, and 42.9%, respectively. A total of 142 UTI pathogens were identified from 150 urine samples.spp. (39%) and(24%) were the major pathogens isolated. Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 93.7%, 75.0%, 91.5%, 93.7%, 88.0%, 72.5%, 13.6%, 44.4%, 71.1%, and 43%, respectively. Twenty-four diarrheal isolates carriedNDM-1 orVIM genes. The overall MBL gene prevalence was 10.5%. Thirty-six UTI pathogens carried eitherNDM-1 orVIM genes (25.4%). Seven isolates carried bothNDM-1 andVIM genes. MBL genes were strongly associated with phenotypic carbapenem and other β-lactam antibiotic resistance.OXA imparted significantly higher phenotypic resistance to β-lactam antibiotics. Active surveillance and stewardship programs are urgently needed to reduce carbapenem resistance in Bangladesh.
Cite this
Shanta, A. S., Islam, N., Al Asad, M., Akter, K., Habib, M. B., Hossain, M. J., Nahar, S., Godman, B., Islam, S. (2024). Resistance and co-resistance of metallo-beta-lactamase genes in diarrheal and urinary-tract pathogens. Microorganisms, 12, 1589.
BibTeX
@article{shanta2024resistance,
title = {Resistance and co-resistance of metallo-beta-lactamase genes in diarrheal and urinary-tract pathogens},
author = {Shanta, A. S. and Islam, N. and Al Asad, M. and Akter, K. and Habib, M. B. and Hossain, M. J. and Nahar, S. and Godman, B. and Islam, S.},
journal = {Microorganisms},
year = {2024},
volume = {12},
pages = {1589},
doi = {10.3390/microorganisms12081589},
}